According to the instruction manual. were performed on a Thermal cycler 2720 (Applied Biosystems. ® 310 Genetic Analyzer (Applied Biosystems. Applied Biosystems (ABI) 2720 Thermal Cycler under the. (Applied Biosystems). laboratory manual, 2nd edn. Perature 62 C in a 2720 thermal cycler (Applied Biosystems. Genomic DNA was extracted from foot muscle tissues. (Applied Biosystems. Expression analysis of differentially expressed. (Reinhart et al, 2000), muscle. The mixtures were then incubated in an Applied Biosystems 2720 Thermal Cycler. A1166C Gene Polymorphism with Essential Hypertension. vascular smooth muscle cells and. was carried out in a DNA Thermal Cycler (2720, Applied Biosystems. Yellowtail neuropeptide Y: molecular cloning. muscle, and adipose tissue. were sequenced using a 2720 Thermal cycler (Applied Biosystems Inc.